Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
STEMCELL Technologies Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum, Size: 500 mL
Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum is recommended for use as a buffer to dilute or wash nucleated cells.
STEMCELL Technologies EasySep™ Buffer, Size: 1 L
EasySep™ Buffer is recommended for EasySep™ cell separation protocols.
Teknova 1M HEPES, pH 7.5 . 1000mL, Sterile, Dnase/Rnase Tested. PCR Grade
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 10X PBS Solution, pH 6.5 (at 1X). 1000 mL, Sterile
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Bioworld Tris-HCl Buffer 10mM, pH 8.5, 500ml
Common buffer for biological sciences. Technical Information: Biotechnology grade, pH 8.5 +-0.1. Warnings: Causes irritation to eyes, skin, and mucus membranes. Do not inhale or ingest. Wash thoroughly after handling. Appearance: Liquid, PH Value: 8.5, Molecular Weight : 157.6, Molecular Formula : C4H11NO3.HCl, pH : 8.5, Solubility : Soluble in water. Appearance Form : Liquid. Appearance Color : Clear. 500ml
Teknova 10X PBS Solution. pH 7.4. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
STEMCELL Technologies RoboSep™ Buffer, Size: 250 mL
RoboSep™ Buffer is recommended for EasySep™ cell separation protocols performed by RoboSep™ . Please note that one or two bottles of buffer are included with every purchase of a RoboSep™ Reagent Kit.
Zymo Research Corporation DNA Wash Buffer (Concentrate), 24 ml
This ensures high purity DNA to be recovered. Ethanol must be added to the concentrate prior to use. Instructions for each DNA Wash Buffer (concentrate) size is listed on the bottle and within the Zymo Research protocols it is used in.
Teknova 20X HBS-EP+ Buffer, pH 7.6.1000mL, Sterile.
3M Sodium Chloride, 200mM HEPES Free Acid,60mM EDTA, 1.0% Tween-20
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (20X) - 500ml
Phosphate Buffered Saline (PBS) is a buffer solution widely used in biological research due to its isotonic and non-toxic nature. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for applications including protein purification, Western Blotting and immunoassays, sample dilution, and as a transport medium. This buffer is formulated in a 20X concentration, and can be diluted to support its application.
Teknova 5M Sodium Chloride Solution. 500 mL, Sterile.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.